i l jensenii i atcc 25258 i  (ATCC)

Journal: Microbial Cell Factories

Article Title: Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion

doi: 10.1186/s12934-024-02612-w

Figure Lengend Snippet: Representative confocal microscopy images of mCherry-labelled lactobacilli L. plantarum , L. gasseri and L. jensenii and GFP-labelled L. crispatus incorporated into PEO nanofibers. Empty PEO nanofibers are shown for comparison

Article Snippet: In this study, we used three vaginal lactobacilli L. crispatus ATCC 33,820, L. gasseri ATCC 33,323, L. jensenii ATCC 25,258 and a control L. plantarum ATCC 8014 to express the fluorescent proteins mCherry and GFP, as previously described [ ], and also engineered these bacteria to express the luminescent protein Nluc.

Techniques: Confocal Microscopy, Comparison

Journal: Microbial Cell Factories

Article Title: Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion

doi: 10.1186/s12934-024-02612-w

Figure Lengend Snippet: Kinetics of release of vaginal lactobacilli from PEO nanofibers determined by measuring fluorescence ( a ), and the influence of electrospinning on the viability of fluorescent lactobacilli ( b ). Percentage of the released bacteria was determined based on the highest overall fluorescent signal. Viability in dispersion was normalized to dry mass to enable comparison with viability in nanofibers. Lpl, Lactiplantibacillus plantarum (red); Lga, Lactobacillus gasseri (brown); Lje, Lactobacillus jensenii (blue); Lcr, Lactobacillus crispatus (green); D, dispersion; N, nanofibers. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student’s t test) relative to bacterial dispersion

Article Snippet: In this study, we used three vaginal lactobacilli L. crispatus ATCC 33,820, L. gasseri ATCC 33,323, L. jensenii ATCC 25,258 and a control L. plantarum ATCC 8014 to express the fluorescent proteins mCherry and GFP, as previously described [ ], and also engineered these bacteria to express the luminescent protein Nluc.

Techniques: Fluorescence, Bacteria, Dispersion, Comparison

Journal: Microbial Cell Factories

Article Title: Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion

doi: 10.1186/s12934-024-02612-w

Figure Lengend Snippet: ( a ) Hemolytic test of bacteria ( Lactiplantibacillus plantarum (LPL); Lactobacillus gasseri (LGA); Lactobacillus jensenii (LJE); Lactobacillus crispatus (LCR)), PEO suspension (S) and PEO nanofibers (NF). Percentage of live Caco-2 cells after 2, 4, 6 and 24 h incubation with bacterial dispersion ( b ) or nanofibers ( c ). Caco-2 cells were incubated with Lactiplantibacillus plantarum (red); Lactobacillus gasseri (brown); Lactobacillus jensenii (blue); Lactobacillus crispatus (green). Controls are shown in black (full line – no addition, dotted line – nanofibers without bacteria). ANOVA with multiple pairwise comparison was performed for each time point. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; relative to control (Caco-2 cells without bacteria or nanofibers)

Article Snippet: In this study, we used three vaginal lactobacilli L. crispatus ATCC 33,820, L. gasseri ATCC 33,323, L. jensenii ATCC 25,258 and a control L. plantarum ATCC 8014 to express the fluorescent proteins mCherry and GFP, as previously described [ ], and also engineered these bacteria to express the luminescent protein Nluc.

Techniques: Bacteria, Suspension, Incubation, Dispersion, Comparison, Control

Journal: Microbial Cell Factories

Article Title: Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion

doi: 10.1186/s12934-024-02612-w

Figure Lengend Snippet: ( a ) Autoaggregation of Lactiplantibacillus plantarum (red); Lactobacillus gasseri (brown); Lactobacillus jensenii (blue); Lactobacillus crispatus (green) monitored over a period of 6 h. Surface hydrophobicity ( b ) of Lactiplantibacillus plantarum (Lpl); Lactobacillus gasseri (Lga); Lactobacillus jensenii (Lje); Lactobacillus crispatus (Lcr) shown as a percentage and determined through extraction with n-hexadecane

Article Snippet: In this study, we used three vaginal lactobacilli L. crispatus ATCC 33,820, L. gasseri ATCC 33,323, L. jensenii ATCC 25,258 and a control L. plantarum ATCC 8014 to express the fluorescent proteins mCherry and GFP, as previously described [ ], and also engineered these bacteria to express the luminescent protein Nluc.

Techniques: Extraction

Journal: Microbial Cell Factories

Article Title: Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion

doi: 10.1186/s12934-024-02612-w

Figure Lengend Snippet: Luminescence intensity of engineered vaginal lactobacilli as a function of bacterial concentration. Lactiplantibacillus plantarum (red circles), Lactobacillus gasseri (brown circles), Lactobacillus jensenii (blue circles), Lactobacillus crispatus (green circles). Non-transformed bacteria (black diamonds) and phosphate-buffered saline (grey triangles) were used as controls

Article Snippet: In this study, we used three vaginal lactobacilli L. crispatus ATCC 33,820, L. gasseri ATCC 33,323, L. jensenii ATCC 25,258 and a control L. plantarum ATCC 8014 to express the fluorescent proteins mCherry and GFP, as previously described [ ], and also engineered these bacteria to express the luminescent protein Nluc.

Techniques: Concentration Assay, Transformation Assay, Bacteria, Saline

Journal: Microbial Cell Factories

Article Title: Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion

doi: 10.1186/s12934-024-02612-w

Figure Lengend Snippet: Luminescence assay of vaginal lactobacilli at different concentrations expressing Nluc. The results are shown as accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and concentration range

Article Snippet: In this study, we used three vaginal lactobacilli L. crispatus ATCC 33,820, L. gasseri ATCC 33,323, L. jensenii ATCC 25,258 and a control L. plantarum ATCC 8014 to express the fluorescent proteins mCherry and GFP, as previously described [ ], and also engineered these bacteria to express the luminescent protein Nluc.

Techniques: Luminescence Assay, Expressing, Concentration Assay, Bacteria

Journal: Microbial Cell Factories

Article Title: Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion

doi: 10.1186/s12934-024-02612-w

Figure Lengend Snippet: Luminescence assay of vaginal lactobacilli expressing Nluc adhered to Caco-2 cells in dispersion or in nanofiber mats. The data are presented as means ± standard deviation (SD)

Article Snippet: In this study, we used three vaginal lactobacilli L. crispatus ATCC 33,820, L. gasseri ATCC 33,323, L. jensenii ATCC 25,258 and a control L. plantarum ATCC 8014 to express the fluorescent proteins mCherry and GFP, as previously described [ ], and also engineered these bacteria to express the luminescent protein Nluc.

Techniques: Luminescence Assay, Expressing, Dispersion, Standard Deviation, Concentration Assay